DNA and RNA Probes: Preparation, Formats, Labeling, Amplification, Detection

August 2011 | 60 pages | ID: D9235D45A11EN
Venture Planning Group

Abstracts

Global PDF: US$ 1,850.00

This comprehensive review of the DNA and RNA probe technology includes modern principles of probe preparation, various test formats, labeling techniques, and numerous amplification methods.

Contains 60 pages

Contents

1. BASIC PRINCIPLES

2. PROBE PREPARATION

3. DNA PROBE TEST

a. Sample Preparation
b. Hybridization
c. Separation
d. Detection/Measurement

4. TEST FORMATS

a. Filter Hybridization
b. Southern Blot
c. Northern Blot
d. In Situ Hybridization
e. Others

5. LABELING TECHNIQUES

6. AMPLIFICATION METHODS

Polymerase Chain Reaction
  - Temperature Cyclers
  - PCR Variations
    * Immuno-PCR
    * QC-PCR
    * DAP-PCR
Strand Displacement Activation
TMA
Ligase Chain Reaction
Branched DNA
  - Hybridization Protection Assay
Nucleic-Acid Sequence-Based Amplification
Self-Sustained Sequence Replicase
Others
  - Ampliprobe
  - CAR
  - CAS
  - CPT
  - Dendritic Polymer Technology
  - ISO-CR
  - LAT
  - Probe Networks
  - RAMP
  - Repair Chain Reaction
  - Rolling Circles
  - Sequence Independent Gene Amplification
  - Sequence Initiation Reaction
  - SISPA